Application of keV and MeV ion microbeams through tapered glass capillaries

Focusing of MeV ion beams by means of tapered glass capillary optics

We present evidence of the focusing effects of fine glass capillary optics for MeV He ion beams. The glass capillary optics are formed by a puller as to have inlet diameters of about 1 mm and outlet diameters of submicrons. The total length of the optics is about 50 mm. Impingent MeV ions to such optics are reflected by the inner wall several times, in a very similar process to the so-called su...

High-Resolution Ion Implantation from keV to MeV

Injection of ultrasmall samples and single molecules into tapered capillaries.

A novel injection scheme is described in which ultrasmall samples in the attoliter (10(-18) L) and low femtoliter (10(-15) L) range, or even single molecules, are controllably introduced into a tapered capillary so that electrophoretic separation can be carried out. To match the dimensions of the capillary inlet with that of the sample, capillary tips are tapered to an inside diameter ranging f...

The design and application of ion microbeams for irradiating living cells and tissues

The range of radiobiological experiments requiring microirradiation techniques continues to expand and diversify, creating ever-greater challenges for the designers of such systems. A versatile microbeam for radiation biology must excel in a number of areas. For studies of intracellular cell signalling where it may be of interest to target just the cytoplasm or nuclear membrane, targeting accur...

A model of thermally generated pH gradients in tapered capillaries

Potential applied at the two ends of a tapered capillary can produce an electric field and a pH gradient at the same time. These fields will effect electrophoretic separation, if the diameters of capillaries are not uniform. In some cases these gradients can be used to our advantage. For example, this approach provides a simple way to perform isoelectric focusing without carrier ampholytes as J...